產品詳情
簡單介紹：
重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白與其它公司提供的重組蛋白不同，rRtKGF-2/FGF-10蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白
| Species | Rat |
| Accession | P70492 |
| GeneID | 25443 |
| Source | Escherichia coli. |
| Molecular Weight | 重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | QALGQDMVSP EATNSSSSSS SSSSSSSFSS PSSAGRHVRS YNHLQGDVRW RKLFSFTKYF LKIEKNGKVS GTKKENCPYS ILEITSVEIG VVAVKAINSN YYLAMNKKGK LYGSKEFNND CKLKERIEEN GYNTYASFNW QHNGRQMYVA LNGKGAPRRG QKTRRKNTSA HFLPMVVHS |
| Purity | 重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 103 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose. |
| Endotoxin | Less than 1 EU/μg of rRtKGF-2/FGF-10 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠角質形成細胞生長因子-2 / FGF-10蛋白 | |
| SDS-PAGE |
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| Reference |
1. Emoto H, Tagashira S, Mattei MG, et al. 1997. J Biol Chem. 272:23191-4. 2. Tagashira S, Harada H, Katsumata T, et al. 1997. Gene. 197:399-404. 3. Carninci P, Kasukawa T, Katayama S, et al. 2005. Science. 309:1559-63. 4. Igarashi M, Finch PW, Aaronson SA. 1998. J Biol Chem. 273:13230-5. |
| Background | Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10. |
