產品詳情
簡單介紹：
重組鼠單核細胞趨化蛋白-2 / CCL8蛋白與其它公司提供的重組蛋白不同，rMuMCP-2/CCL8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠單核細胞趨化蛋白-2 / CCL8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠單核細胞趨化蛋白-2 / CCL8蛋白
| Synonyms | Small-inducible Cytokine A8 |
| Species | Murine |
| Accession | Q9Z121 |
| GeneID | 20307 |
| Source | 重組鼠單核細胞趨化蛋白-2 / CCL8蛋白Escherichia coli. |
| Molecular Weight | Approximately 8.5 kDa, a single, non-glycosylated polypeptide chain containing 74 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | GPDKAPVTCC FHVLKLKIPL RVLKSYERIN NIQCPMEAVV FQTKQGMSLC VDPTQKWVSE YMEILDQKSQ ILQP |
| Purity | 重組鼠單核細胞趨化蛋白-2 / CCL8蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | 重組鼠單核細胞趨化蛋白-2 / CCL8蛋白Less than 1 EU/μg of rMuMCP-2/CCL8 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠單核細胞趨化蛋白-2 / CCL8蛋白 | |
| Reference |
1. Van Coillie E, Fiten P, Nomiyama H, et al. 1997. Genomics. 40:323-31. 2. Opdenakker G, Froyen G, Fiten P, et al. 1993. Biochem Biophys Res Commun. 191:535-42. 3. Proost P, Wuyts A, Van Damme J. 1996. J Leukoc Biol. 59:67-74. 4. Gong W, Howard OM, Turpin JA, et al. 1998. J Biol Chem. 273:4289-92. |
| Background | Murine CCL8, also known as monocyte chemotactic protein 2 (MCP-2), is belonging to the CC chemokine family and is encoded by the gene CCL8 in mouse. MCP-2 has two homogeneous MCP-1 (CCL2) and MCP-3 (CCL7). These three MCPs were found by IL-1-beta triggered human MG-63 osteosarcoma cells. CCL8 shares 62 % amino acid sequence identity with MCP-1, and shares 58 % amino acid sequence identity with MCP-2. CCL8 has chemotactic fuction for monocytes, eosinophils and neutrophils. In addition, it can also chemoattract activated NK cells. |
