產品詳情
簡單介紹：
重組人血小板因子-4 / CXCL4蛋白與其它公司提供的重組蛋白不同，rHuPF-4/CXCL4蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人血小板因子-4 / CXCL4蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人血小板因子-4 / CXCL4蛋白
| Synonyms | Iroplact, Oncostatin-A |
| Species | Human |
| Accession | P02776 |
| GeneID | 5196 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人血小板因子-4 / CXCL4蛋白Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 70 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | EAEEDGDLQC LCVKTTSQVR PRHITSLEVI KAGPHCPTAQ LIATLKNGRK ICLDLQAPLY KKIIKKLLES |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human fibroblasts is in a concentration of 1.0-10 ng/ml. |
| Physical Appearance | 重組人血小板因子-4 / CXCL4蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, 1.5 M NaCl, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuPF-4/CXCL4 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20°C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人血小板因子-4 / CXCL4蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人血小板因子-4 / CXCL4蛋白 | |
| Reference |
1. O'Donovan N, Galvin M, Morgan JG. 1999. Cytogenet Cell Genet. 84:39-42. 2. Eisman R, Surrey S, Ramachandran B, et al. 1990. Blood. 76:336-44. 3. Lasagni L, Francalanci M, Annunziato F, et al. 2003. J Exp Med. 197:1537-49. 4. Warkentin TE. 2007. N Engl J Med. 356:891-3. |
| Background | Platelet Factor-4 (PF-4) is a small cytokine belonging to the CXC chemokine family that is also known as chemokine (C-X-C motif) ligand 4 (CXCL4). The gene for human CXCL4 is located on human chromosome 4. This chemokine is released from alpha-granules of activated platelets during platelet aggregation and neutralizes the anticoagulant effect of heparin. It interacts with a splice variant of the chemokine receptor CXCR3 besides be chemotactic for neutrophils, fibroblasts and monocytes. Recombinant human CXCL4 contains 70 amino acids binds with high affinity to heparin. Specifically, Human and mouse CXCL4 share about a 60 % identity. |
