產品詳情
簡單介紹：
重組大鼠制瘤蛋白-M蛋白與其它公司提供的重組蛋白不同，rRtOSM蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組大鼠制瘤蛋白-M蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組大鼠制瘤蛋白-M蛋白
| Species | Rat |
| Accession | Q65Z15 |
| GeneID | 289747 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 24.3 kDa, a single non-glycosylated polypeptide chain containing 214 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | 重組大鼠制瘤蛋白-M蛋白KRGCSSSSPK LLSQLKSQAN ITGNTASLLE PYILHQNLNT LTLRAACTEH PVAFPSEDML RQLSKPDFLS TVHATLGRVW HQLGAFRQQF PKIQDFPELE RARQNIQGIR NNVYCMARLL HPPLEIPEPT QADSGTSRPT TTAPGIFQIK IDSCRFLWGY HRFMGSVGRV FEEWGDGSRR SRRHSPLWAW LKGDHRIRPS RSSQSAMLRS LVPR |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組大鼠制瘤蛋白-M蛋白Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine NIH-3T3 cells is less than 2.0 ng/ml, corresponding to a specific activity of > 5.0 × 105 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, 0.1 % Tween-80. |
| Endotoxin | Less than 1 EU/μg of rRtOSM as determined by LAL method. |
| Reconstitution | 重組大鼠制瘤蛋白-M蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組大鼠制瘤蛋白-M蛋白 | |
| Reference |
1. Tanaka M, Miyajima A. 2003. Rev Physiol Biochem Pharmacol. 149:39-52. 2. Mosley B, De Imus C, Friend D, et al. 1996. J Biol Chem. 271:32635-43. 3. Malik N, Kallestad JC, Gunderson NL, et al. 1989. Mol Cell Biol. 9:2847-53. 4. Brown TJ, Rowe JM, Liu JW, et al. 1991. J Immunol. 147:2175-80. |
| Background | Oncostatin-M (OSM) is a multifunctional cytokine that belongs to the Interleukin-6 subfamily. Among the family members, OSM is most closely related to leukemia inhibitory factor (LIF) and it in fact utilizes the LIF receptor in addition to its specific receptor in the human. A biologically active OSM receptor has been previously described that consists of a heterodimer of leukemia inhibitory factor receptor (LIFR) and gp130. OSM is synthesized by stimulated T-cells and monocytes. Furthermore, the effects of OSM on endothelial cells suggest a pro-inflammatory role for OSM and endothelial cells possess a large number of OSM receptors. Recombinant murine OSM contains 215 amino acids and has a molecular mass of 22.4 kDa. It has approximately 48 % and 72 % amino acid sequence identity with human and murine OSM. |
