產品詳情
簡單介紹：
重組鼠胸腺表達趨化因子/ CCL25蛋白與其它公司提供的重組蛋白不同，rMuTECK/CCL25蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠胸腺表達趨化因子/ CCL25蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠胸腺表達趨化因子/ CCL25蛋白
| Synonyms | SCYA25, Ckb15 |
| Species | Murine |
| Accession | O35903 |
| Unigene | Mm.7275. |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠胸腺表達趨化因子/ CCL25蛋白Approximately 14.1 kDa, a single, non-glycosylated polypeptide chain containing 121 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | QGAFEDCCLG YQHRIKWNVL RHARNYHQQE VSGSCNLRAV RFYFRQKVVC GNPEDMNVKR AIRILTARKR LVHWKSASDS QTERKKSNHM KSKVENPNST SVRSATLGHP RMVMMPRKTN N |
| Purity | 重組鼠胸腺表達趨化因子/ CCL25蛋白> 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 5.0-50 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | 重組鼠胸腺表達趨化因子/ CCL25蛋白Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rMuTECK/CCL25 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠胸腺表達趨化因子/ CCL25蛋白 | |
| SDS-PAGE |
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| Reference |
1. Uehara S, Song K, Farber JM, et al. 2002. J Immunol, 168: 134-42. 2. Ericsson A, Svensson M, Arya A, et al. 2004. Eur J Immunol, 34: 2720-9. 3. Svensson MandAgace WW. 2006. Expert Rev Clin Immunol, 2: 759-73. |
| Background | CCL25 is new member of CC family chemokine. It is also called Thymus-expressed chemokine (TECK) because it is restricted produced by thymus and intestine. Especially, the dendritic cells derived from thymus but not bone marrow had identified to be the source of CCL25. By binding with CCR9, it elicits its effects of chemotactic for thymocytes, macrophages, and dendritic cells. Additionally, CCL25 takes part in regulating the development of T-cells. |
