產(chǎn)品詳情
簡單介紹：
重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白與其它公司提供的重組蛋白不同，rMuMIP-3β/CCL19蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白
| Synonyms | Beta-chemokine Dxodus-3, CK beta-11, EBI1 Ligand Dhemokine, ELC Macrophage Inflammatory Protein 3 beta |
| Species | Murine |
| Accession | O70460 |
| GeneID | 24047 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白Approximately 9.2 kDa, a single non-glycosylated polypeptide chain containing 83 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | GANDAEDCCL SVTQRPIPGN IVKAFRYLLN EDGCRVPAVV FTTLRGYQLC APPDQPWVDR IIRRLKKSSA KNKGNSTRRS PVS |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human mature dendritic cells is in a concentration range of 10-100 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | 重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白Less than 1 EU/μg of rMuMIP-3β/CCL19 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠巨噬細(xì)胞炎癥蛋白-3 beta / CCL19蛋白 | |
| Reference |
1. Milicevic NM, Miljkovic MD, Milicevic Z, et al. 2011. Histochem Cell Biol, 135: 593-601. 2. Guerin LR, Moldenhauer LM, Prins JR, et al. 2011. Biol Reprod, 85: 397-408. 3. Yoshida R, Imai T, Hieshima K, et al. 1997. J Biol Chem, 272: 13803-9. 4. Gibejova A, Mrazek F, Subrtova D, et al. 2003. Am J Respir Crit Care Med, 167: 1695-703. |
| Background | CCL19, also known as EBI1 Ligands chemokine (ELC) and macrophage inflammatory protein-3-beta, is encoded by the CCL19 gene which is located on chromosome 4 in mouse. The protein is expressed at high levels in thymus and lymph nodes, with intermediate levels in trachea and colon and low levels in stomach, lung and some other organs. CCL19 is signal through CCR7 and chemotaxis of T and B lymphocytes and myeloid progenitor cells. |
