產品詳情
簡單介紹：
重組鼠SF-20 / IL-25蛋白與其它公司提供的重組蛋白不同，rMuSF-20/IL-25蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠SF-20 / IL-25蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠SF-20 / IL-25蛋白
| Synonyms | IL-17E, IL-25 |
| Species | Murine |
| Accession | Q9CPT4 |
| GeneID | 28106 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠SF-20 / IL-25蛋白Approximately 15.8 kDa, a single non-glycosylated polypeptide chain containing 143 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠SF-20 / IL-25蛋白Data Not Available. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuSF-20 as determined by LAL method. |
| Reconstitution | 重組鼠SF-20 / IL-25蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠SF-20 / IL-25蛋白 | |
| SDS-PAGE |
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| Reference |
1. Tulin EE, Onoda N, Nakata Y, et al. 2003. J Immunol, 170: 1593. 2. Terashima A, Watarai H, Inoue S, et al. 2008. J Exp Med, 205: 2727-33. |
| Background | SF-20 or IL-25 belongs to the IL-17 family and is also named IL-17E. It is encoded by IL-25 (IL-17E) gene located on chromosome 14 in murine and secreted by several tissues at low levels, for instance, brain, kidney, lung, prostate, testis, spinal cord, adrenal gland, and trachea. This cytokine is initially identified as a product of bone marrow-derived stromal cells and plays an important role in proliferation of lymphoid cells and is considered an interleukin. It rest CD8+ and CD19+ cells and activated CD8+ T cells and has been shown to bind to the surface of cells expressing the receptor TSA-1 (Thymic shared Ag-1). Additionally, it induces the production of other cytokines, including IL-4, IL-5 and IL-13 in multiple tissues, which stimulate the expansion of eosinophils. |
