產品詳情
簡單介紹：
重組人類Cu / Zn超氧化物歧化酶蛋白與其它公司提供的重組蛋白不同，rHuCu/Zn SOD蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人類Cu / Zn超氧化物歧化酶蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人類Cu / Zn超氧化物歧化酶蛋白
| Synonyms | SOD1 |
| Species | Human |
| Accession | P00441 |
| GeneID | 6647 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人類Cu / Zn超氧化物歧化酶蛋白Approximately 31.6 kDa, a homodimer, non-glycosylated polypeptide chain containing 2 × 153 amino acids. |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | ATKAVCVLKG DGPVQGIINF EQKESNGPVK VWGSIKGLTE GLHGFHVHEF GDNTAGCTSA GPHFNPLSRK HGGPKDEERH VGDLGNVTAD KDGVADVSIE DSVISLSGDH CIIGRTLVVH EKADDLGKGG NEESTKTGNA GSRLACGVIG IAQ |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人類Cu / Zn超氧化物歧化酶蛋白Fully biologically active when compared to standard. The potency per mg was determined by Pyrogallic Acid method and was found to be more than 1.0 × 104 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuCu/Zn SOD as determined by LAL method. |
| Reconstitution | 重組人類Cu / Zn超氧化物歧化酶蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人類Cu / Zn超氧化物歧化酶蛋白 | |
| SDS-PAGE |
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| Reference |
1. Kwiatowski J, Skarecky D, Ayala FJ. 1992. Mol Phylogenet Evol, 1: 72-82. 2. Bachus R, Claus A, Megow D, et al. 1995. J Neurol Sci, 129 Suppl: 93-5. 3. Qi X, Guy J, Nick H, et al. 1997. Invest Ophthalmol Vis Sci, 38: 1203-12. 4. Chou CM, Huang CJ, Shih CM, et al. 2005. Ann N Y Acad Sci, 1042: 303-13. 5. Raja SB, Murali MR, Roopa K, et al. 2011. Biomed Pharmacother, 65: 560-8. |
| Background | Superoxide dismutase catalyzes the reaction between superoxide anions and hydrogen to yield molecular oxygen and hydrogen peroxide. Cu/Zn superoxide dismutase also named as SOD1, is an enzyme encoded by the SOD1 gene in humans, located on chromosome 21. The SOD1 binds Cu and Zn ions and is one of three SODs responsible for destroying free superoxide radicals in the body. It has been shown to interact with CCS and Bcl-2. The malfunction of SOD1 may increase the risk of illnesses like age-related muscle mass loss (sarcopenia), early development of cataracts, macular degeneration, thymic involution, hepatocellular carcinoma, shortened lifespan, keratoconus and amyotrophic lateral sclerosis. |
