產品詳情
簡單介紹：
重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白與其它公司提供的重組蛋白不同，rHuMIP-1α/CCL3蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白
| Synonyms | G0/G1 switch regulatory protein 19-1, MIP-1-alpha, PAT 464.1, SIS-beta, Small-inducible cytokine A3,Tonsillar lymphocyte LD78 alpha protein |
| Species | Human |
| Accession | P10147 |
| GeneID | 6348 |
| Source | Escherichia coli. |
| Molecular Weight | 重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白Approximately 7.8 kDa protein containing 70 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | ASLAADTPTA CCFSYTSRQI PQNFIADYFE TSSQCSKPGV IFLTKRSRQV CADPSEEWVQ KYVSDLELSA |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human monocytes is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuMIP-1α/CCL3 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人巨噬細胞炎癥蛋白-1 alpha / CCL3蛋白 | |
| SDS-PAGE |
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| Reference |
1. Guan E, Wang J, Norcross MA. 2001. J Biol Chem. 276:12404-9. 2. Cocchi F, DeVico AL, Garzino-Demo A, et al. 1995. Science. 270:1811-5. 3. Nakao M, Nomiyama H, Shimada K. 1990. Mol Cell Biol. 10:3646-58. 4. Czaplewski LG, McKeating J, Craven CJ, et al. 1999. J Biol Chem. 274:16077-84. 5. 1991. Hum Gene Ther. 2:87-96. |
| Background | CCL3, also known as macrophage inflammatory protein-1α (MIP-1α), is a cytokine belonging to the CC chemokine family that is involved in the acute inflammatory state in the recruitment and activation of polymorphonuclear leukocytes. Studies showed CCL3 can bind to CCR1, CCR4 and CCR5. MIP-1α can induce a dose-dependent inhibition of different strains of HIV-1, HIV-2, and simian immunodeficiency virus (SIV). Recombinant human MIP-1α is a 7.8 kDa protein containing 69 amino acid residues, including the four highly conserved cysteine residues present in CC chemokines and it shares approximately 73 %, 74 % and 92 % amino acid sequence homology with the murine, rat and rhesus macaque protein. |
