產品詳情
簡單介紹：
重組蛋白價格與其它公司提供的重組蛋白不同，蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組重組蛋白價格可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
| Synonyms | Dendritic Cell and Monocyte Chemokine-like Protein, DMC |
| Species | Rat |
| Source | Escherichia coli. |
| Molecular Weight | 重組蛋白價格Approximately 11.5 kDa, a single non-glycosylated polypeptide chain containing 97 amino acids. |
| Quantity | 2μg/10μg/1000μg |
| AA Sequence | SPNQEVARHH GDQHQAPRRW LWEGGQECDC KDWSLRVSKR KTTAVLEPPR KQCPCDHVKG SEKKNRRQKH HRKSQRPSRT CQQFLKRCQL ASFTLPL |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組蛋白價格Fully biologically active when compared to standard. The ED50 as determined by its ability to induce VEGF expression using murine endothelial cells is less than 5.0 μg/ml, corresponding to a specific activity of > 200 IU/mg. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 2 × PBS, pH 7.4. |
| Endotoxin | 重組蛋白價格Less than 1 EU/μg of rRtVCC-1/CXCL17 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組蛋白價格 | |
| Reference |
1. WY Lee, CJ Wang, TY Lin, et al. 2013. Am J Physiol Endocrinol Metab, 304: E32-40. 2. A Matsui, H Yokoo, Y Negishi, et al. 2012. PLoS One, 7: e44080. 3. AM Burkhardt, KP Tai, JP Flores-Guiterrez, et al. 2012. J Immunol, 188: 6399-406. 4. N Hiraoka, R Yamazaki-Itoh, Y Ino, et al. 2011. Gastroenterology, 140: 310-21. |
| Background | 重組蛋白價格also named Dendritic Cell and Monocyte Chemokine-like Protein (DMC) and VEGF Co-regulated Chemokine 1 (VCC-1), is a small cytokine belonging to the CXC chemokine family. It is constitutively expressed in the lung and can be detected in trachea, stomach and skeletal muscle. CXCL17 attracts dendritic cells and monocytes and is regulated in tumors. It may be a housekeeping chemokine regulating recruitment of nonactivated blood monocytes and immature dendritic cells into tissues and may play a role in the innate defense against infections. Mature human CXCL17 shares 73 %, 71 % and 64 % amino acid sequence identity with bovine, mouse and rat DMC, respectively. |
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