產品詳情
簡單介紹：
重組鼠音速刺猬蛋白與其它公司提供的重組蛋白不同，rMuSHH蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠音速刺猬蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠音速刺猬蛋白
| Synonyms | SHH, HHG-1 |
| Species | Murine |
| Accession | Q62226 |
| GeneID | 20423 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠音速刺猬蛋白Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 176 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | IVIGPGRGFG KRRHPKKLTP LAYKQFIPNV AEKTLGASGR YEGKITRNSE RFKELTPNYN PDIIFKDEEN TGADRLMTQR CKDKLNALAI SVMNQWPGVK LRVTEGWDED GHHSEESLHY EGRAVDITTS DRDRSKYGML ARLAVEAGFD WVYYESKAHI HCSVKAENSV AAKSGG |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠音速刺猬蛋白Fully biologically active when compared to standard. The ED50 as determined by inducing alkaline phosphatase production of murine C3H10T1/2 cells is 0.5 - 1.0 μg/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rMuSHH as determined by LAL method. |
| Reconstitution | 重組鼠音速刺猬蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組鼠音速刺猬蛋白 | |
| SDS-PAGE |
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| Reference |
1. Currie PD, Ingham PW. 1996. Nature. 382:452-5. 2. Herzog W, Zeng X, Lele Z, et al. 2003. Dev Biol. 254:36-49. 3. Lewis KE, Eisen JS. 2001. Development. 128:3485-95. 4. Scholpp S, Wolf O, Brand M, et al. 2006. Development. 133:855-64. 5. Dassule HR, Lewis P, Bei M, et al. 2000. Development. 127:4775-85. 6. Porter JA, Young KE, Beachy PA. 1996. Science. 274:255-9. |
| Background | Sonic hedgehog is one of three proteins in the mammalian signaling pathway family called hedgehog, the others being desert hedgehog (DHH) and indian hedgehog (IHH). As the hh homologues, SHH has been found to have the most critical roles in development. It acts as a morphogen involved in patterning many systems, including the limb and midline structures in the brain, spinal cord, the thalamus by the zona limitans intrathalamica and the teeth. There SHH undergoes auto-processing to generate a 20 kDa (residues 24 - 197) N-terminal signaling domain (SHH-N) and a 25 kDa (residues 198 - 462) C-terminal domain with no known signaling role. |
