產品詳情
簡單介紹：
重組小鼠成纖維細胞生長因子8蛋白與其它公司提供的重組蛋白不同，rMuFGF-8蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組小鼠成纖維細胞生長因子8蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組小鼠成纖維細胞生長因子8蛋白
| Synonyms | AIGF, HBGF-8, FGF-8c |
| Species | Murine |
| Accession | P37237 |
| GeneID | 14179 |
| Source | Escherichia coli. |
| Molecular Weight | 重組小鼠成纖維細胞生長因子8蛋白Approximately 28.1 kDa, a single non-glycosylated polypeptide chain containing 246 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | QVRSAAQKRG PGAGNPADTL GQGHEDRPFG QRSRAGKNFT NPAPNYPEEG SKEQRDSVLP KVTQRHVREQ SLVTDQLSRR LIRTYQLYSR TSGKHVQVLA NKRINAMAED GDPFAKLIVE TDTFGSRVRV RGAETGLYIC MNKKGKLIAK SNGKGKDCVF TEIVLENNYT ALQNAKYEGW YMAFTRKGRP RKGSKTRQHQ REVHFMKRLP RGHHTTEQSL RFEFLNYPPF TRSLRGSQRT WAPEPR |
| Purity | 重組小鼠成纖維細胞生長因子8蛋白> 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using murine balb/c 3T3 cells is less than 5.0 ng/ml, corresponding to a specific activity of > 2.0 × 105 IU/mg in the presence of 10 μg/ml of heparin. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4, 500 mM NaCl. |
| Endotoxin | 重組小鼠成纖維細胞生長因子8蛋白Less than 1 EU/μg of rMuFGF-8 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組小鼠成纖維細胞生長因子8蛋白 | |
| SDS-PAGE |
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| Reference |
1. Powers CJ, McLeskey SW, Wellstein A. 2000. Endocr Relat Cancer. 7:165-97. 2. Mattila MM, Harkonen PL. 2007. Cytokine Growth Factor Rev. 18:257-66. 3. Tanaka A, Miyamoto K, Minamino N, et al. 1992. Proc Natl Acad Sci U S A. 89:8928-32. 4. Gemel J, Gorry M, Ehrlich GD, et al. 1996. Genomics. 35:253-7. 5. Blunt AG, Lawshe A, Cunningham ML, et al. 1997. J Biol Chem. 272:3733-8. 6. Ornitz DM, Xu J, Colvin JS, et al. 1996. J Biol Chem. 271:15292-7. 7. Zhang X, Ibrahimi OA, Olsen SK, et al. 2006. J Biol Chem. 281:15694-700. 8. Olsen SK, Li JY, Bromleigh C, et al. 2006. Genes Dev. 20:185-98. |
| Background | Murine FGF-8 is a heparin binding growth factor belonging to the FGF family, which plays a central role during prenatal development, postnatal growth and regeneration of a variety of tissues, by promoting cellular proliferation and differentiation. Murine FGF-8 is first purified from an androgen-dependent mouse mammary carcinoma cell line as an androgen induces secretion. Cloning and analysis of the murine FGF8 gene revealed at least eight potential protein isoforms (FGF-8a-h). Murine FGF-8a and b share 100 % amino acid identity with that in humans, and murine FGF-8e and f share 98 % amino acid identity with humans. None of the FGF-8 isoforms exhibited activity to FGFR1b, 2b, 3b, but FGFR2c, 3c and FGFR4 can be activated by several FGF-8 isoforms. FGF-8 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration, and it is required for normal brain, eye, ear and limb development during embryogenesis. |
