產品詳情
簡單介紹：
重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白與其它公司提供的重組蛋白不同，rMuMIP-2/CXCL2蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白
| Species | Murine |
| Accession | P10889 |
| GeneID | 20310 |
| Source | Escherichia coli. |
| Molecular Weight | 重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白Approximately 7.8 kDa, a single, non-glycosylated polypeptide chain containing 73 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | AVVASELRCQ CLKTLPRVDF KNIQSLSVTP PGPHCAQTEV IATLKGGQKV CLDPEAPLVQ KIIQKILNKG KAN |
| Purity | > 97 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human neutrophils is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20 mM PB, pH 7.4, 150 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rMuMIP-2/CXCL2 as determined by LAL method. |
| Reconstitution | 重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
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重組鼠巨噬細胞炎癥蛋白-2 / CXCL2蛋白 |
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| Reference |
1. Haskill S, Peace A, Morris J, et al. 1990. Proc Natl Acad Sci U S A. 87:7732-6. 2. Tsai HH, Frost E, To V, et al. 2002. Cell. 110:373-83. 3. Wolpe SD, Sherry B, Juers D, et al. 1989. Proc Natl Acad Sci U S A. 86:612-6. 4. Iida N, Grotendorst GR. 1990. Mol Cell Biol. 10:5596-9. 5. Pelus LM, Fukuda S. 2006. Exp Hematol. 34:1010-20. |
| Background | Murine CXCL2, also named MIP-2, is belonging to the CXC chemokine family. It is encoded by the gene CXCL2. CXCL2 shares 90 % amino acid sequence with CXCL1/GROα. It is member of the intercrine alpha (chemokine C-X-C) subfamily of chemokines. This chemokine is secreted from a murine macrophage cell line. The functional receptor for CXCL2 has been identified as CXCR2. CXCL2 is chemotactic for polymorphonuclear leukocytes and hematopoietic stem cells. Similar to other GRO proteins, CXCL2 is potent neutrophil attractants and activators. In addition, it is also active toward basophils. The amino acid sequence of murine CXCL2 is 60 % identical to human CXCL2. |
