產(chǎn)品詳情
簡單介紹：
重組人內(nèi)皮抑素蛋白與其它公司提供的重組蛋白不同，rHuEndostatin蛋白產(chǎn)品為采用CFS的無細(xì)胞麥胚蛋白合成系統(tǒng)表達(dá)出來的重組蛋白，可表達(dá)出對細(xì)胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術(shù)則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人內(nèi)皮抑素蛋白（全長序列）產(chǎn)品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人內(nèi)皮抑素蛋白
| Species | Human |
| Accession | P39059 |
| GeneID | 1306 |
| Source | Escherichia coli. |
| Molecular Weight | Approximately 19.8 kDa, a single non-glycosylated polypeptide chain containing 177 amino acids (C-terminal fragment of collagen XVIII). |
| Quantity | 20μg/100μg/1000μg |
| AA Sequence | 重組人內(nèi)皮抑素蛋白NYEKPALHLA ALNMPFSGDI RADFQCFKQA RAAGLLSTYR AFLSSHLQDL STIVRKAERY SLPIVNLKGQ VLFNNWDSIF SGHGGQFNMH IPIYSFDGRD IMTDPSWPQK VIWHGSSPHG VRLVDNYCEA WRTADTAVTG LASPLSTGKI LDQKAYSCAN RLIVLCIENS FMTDARK |
| Purity | > 96 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | Fully biologically active when compared to standard. The ED50 as determined by inhibiting the FGF basic-dependent proliferation of HUVEC migration is less than 2.0 μg/mL in the presence of Anti-Human Endostatin Polyclonal Antibody. |
| Physical Appearance | 重組人內(nèi)皮抑素蛋白Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuEndostatin as determined by LAL method. |
| Reconstitution | 重組人內(nèi)皮抑素蛋白We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組人內(nèi)皮抑素蛋白 | |
| Reference |
1. HT Ren, H Hu, Y Li, et al. 2013. J Zhejiang Univ Sci B, 14: 224-30. 2. W Zhuo, Y Chen, X Song, et al. 2011. Front Med, 5: 336-40. 3. G HuangandL Chen. 2009. Cancer Biother Radiopharm, 24: 589-96. 4. DM Wei, Y Gao, XR Cao, et al. 2006. Biochem Biophys Res Commun, 345: 1398-404. 5. B Brankin, M Campbell, P Canning, et al. 2005. Exp Eye Res, 81: 22-31. |
| Background | Endostatin has been identified as a C-terminal fragment of Collagen type 18, a recently identified member of a family of collagen-like proteins referred to as multiplexin family. Endostatin specifically inhibits proliferation of endothelial cells although it does not affect the proliferation of EOMA cells. Endostatin also potently inhibits angiogenesis and tumor growth. Endostatin has an important role in endothelial cell adhesion and cytoskeletal organization. Endostatin can be found in vessel walls (elastic fibers) and basement membranes. Recombinant Endosatin expressed in yeast causes G1 arrest of endothelial cells, and endostatin treatment results in apoptosis of HUVE and HMVE cells. |
