產品詳情
簡單介紹：
重組人調節激活正常T細胞快速序列/ CCL5蛋白與其它公司提供的重組蛋白不同，rHuRANTES/CCL5蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組人調節激活正常T細胞快速序列/ CCL5蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組人調節激活正常T細胞快速序列/ CCL5蛋白
| Synonyms | SIS-delta, Small-inducible cytokine A5, T-cell-specific protein RANTES |
| Species | Human |
| Accession | P13501 |
| GeneID | 6352 |
| Source | 重組人調節激活正常T細胞快速序列/ CCL5蛋白Escherichia coli. |
| Molecular Weight | Approximately 7.8 kDa, a single non-glycosylated polypeptide chain containing 68 amino acids. |
| Quantity | 5μg/20μg/1000μg |
| AA Sequence | SPYSSDTTPC CFAYIARPLP RAHIKEYFYT SGKCSNPAVV FVTRKNRQVC ANPEKKWVRE YINSLEMS |
| Purity | > 98 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組人調節激活正常T細胞快速序列/ CCL5蛋白Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using human peripheral blood monocytes is in a concentration range of 1.0-10 ng/ml. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in 20mM PB, pH 7.4, 100 mM NaCl. |
| Endotoxin | Less than 1 EU/μg of rHuRANTES/CCL5 as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| 重組人調節激活正常T細胞快速序列/ CCL5蛋白 | |
| SDS-PAGE |
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| Reference |
重組人調節激活正常T細胞快速序列/ CCL5蛋白1. Hedrick JA, Helms A, Vicari A, et al. 1998. Blood, 91: 4242-7. 2. Nomiyama H, Fukuda S, Iio M, et al. 1999. J Interferon Cytokine Res, 19: 227-34. 3. Youn BS, Zhang S, Broxmeyer HE, et al. 1998. Biochem Biophys Res Commun, 247: 217-22. 4. Kim IS, Jang SW, Sung HJ, et al. 2005. FEBS Lett, 579: 6044-8. 5. Francica G, Petrolati A, Di Stasio E, et al. 2012. Acta Radiol, 53: 394-400. |
| Background | Chemokine (C-C motif) ligand 5 (CCL5) also known as RANTES is classified as a chemotactic cytokine or chemokine. It is encoded by the CCL5 gene in humans. CCL5 is chemotactic for T cells, eosinophils, and basophils, and plays an active role in recruiting leukocytes into inflammatory sites which has been reported to be produced by renal tubular epithelium, synovial fibroblasts and selected tumor cells. It also induces the proliferation and activation of certain natural-killer cells to form CHAK cells. Recombinant human CCL5 contains 68 amino acids and it shares 84 % a.a. sequence identity with murine and rat CCL5, respectively. |
