產品詳情
簡單介紹：
重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白與其它公司提供的重組蛋白不同，rHuTWEAK Receptor/TNFRSF12A蛋白產品為采用CFS的無細胞麥胚蛋白合成系統表達出來的重組蛋白，可表達出對細胞有毒性、易被蛋白酶降解的蛋白；并獲得具有良好的可溶性，并有翻譯后修飾、從而部分具有功能的蛋白.同時獨有的全自動蛋白純化技術則簡便高效，將蛋白純化過程中對蛋白的損傷降低到*小程度.重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白（全長序列）產品可用于Western Blot驗證、抗體制備、蛋白檢測、ELISA等試驗中.
詳情介紹：
重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白
| Synonyms | TNFRSF12A, FGF-inducible 14, CD266 |
| Species | Human |
| Accession | Q9NP84 |
| GeneID | 51330 |
| Source | Escherichia coli. |
| Molecular Weight | 重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白Approximately 5.6 kDa, a single non-glycosylated polypeptide chain containing 53 amino acids. |
| Quantity | 5μg/25μg/1000μg |
| AA Sequence | EQAPGTAPCS RGSSWSADLD KCMDCASCRA RPHSDFCLGC AAAPPAPFRL LWP |
| Purity | > 95 % by SDS-PAGE and HPLC analyses. |
| Biological Activity | 重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白Fully biologically active when compared to standard. The ED50 as determined by inhibiting TWEAK-dependent proliferation of human umbilical vein endothelial cells (HUVEC) is less than 30 ng/ml, corresponding to a specific activity of > 3.3 × 104 IU/mg, in the presence of 15 ng/ml of rHuTWEAK. |
| Physical Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Formulation | Lyophilized from a 0.2 μm filtered concentrated solution in PBS, pH 7.4. |
| Endotoxin | Less than 1 EU/μg of rHuTWEAK Receptor as determined by LAL method. |
| Reconstitution | We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤ -20 °C. Further dilutions should be made in appropriate buffered solutions. |
| Storage | 重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白This lyophilized preparation is stable at 2-8 °C, but should be kept at -20 °C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8 °C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20 °C to -70 °C. Avoid repeated freeze/thaw cycles. |
| 重組體人TNF-related弱誘導細胞凋亡受體/ TNFRSF12A蛋白 | |
| Reference |
1. Gaudineau B, Fougere M, Guaddachi F, et al. 2012. J Cell Sci, 125: 4475-86. 2. Abend JR, Uldrick T, Ziegelbauer JM. 2010. J Virol, 84: 12139-51. 3. Kang S, Yang C, Luo R. 2008. Biochem Biophys Res Commun, 372: 629-33. 4. Wiley SRandWinkles JA. 2003. Cytokine Growth Factor Rev, 14: 241-9. |
| Background | Human TNF-related weak inducer of apoptosis receptor (TWEAKR) also known as Tumor necrosis factor receptor superfamily member 12A precursor (gene name TNFRSF12A) or fibroblast growth factor-inducable 14 kD protein, is distantly related to the TNFR family, containing one cysteine-rich domain in the extracellular region and a TNFR-associated factor binding domain but does not contain a death domain (DD) cytoplasmic region. It is expressed in the spleen, thymus, peripheral blood lymphocytes, colon, and small intestine. Signal transduction by TWEAK receptor can be activated by either the membrane anchored or the soluble TWEAK. In addition, It plays a role in TWEAK-induced endothelial cell migration, proliferation, and angiogenesis. Human and mouse TWEAK R share 82 % a.a. sequence identity. |
